Effect of engineered superparamagnetic iron oxide nanoparticles in targeted cardiac precursor cell delivery by MRI (February 2021)

• The cardiac specific mAb receptors are bound to SPION-PEG micelle through covalent bonding.

• The engineered cardiac specific nanoprobe when incubated with the MSCs of stromal vascular fraction yields positively selected cells through the magnetic assorted cell sorting column.

• Myocardial infarction is introduced in the healthy mice using isoproterenol through the intraperitoneal route. Later GloTrack labelled cardiac precursors are administered.

• Visualisation of the stem cells is done using magnetic resonance imaging (MRI) and functional studies through echocardiogram (ECG).

A scientific approach is presented describing the fabrication of nanoprobe (GloTrack) that can act as cardiac precursor label to segregate cells from cardiac/non cardiac origins and traced by magnetic resonance imaging (MRI). Signal regulatory protein alpha (SIRPA) and kinase domain receptor (KDR) recognizing antibodies, form a layer on super paramagnetic iron oxide nanoparticle – poly-ethylene glycol (SPION-PEG) complex, and bind to protein expressed on the surface of cardiac muscle cells. Physical attributes size, distribution, labelling efficiency, echocardiogram (ECG) changes and bio-distribution by MRI were analysed. The results indicate that GloTrack has an average size of 471 nm, exhibits negative potential and promotes labelling efficiency. The bio-distribution of GloTrack in in vivo experiments was traceable in 7T MRI showing high accumulation of GloTrack in cardiac muscles as compared to the liver and spleen. ECG data revealed that GloTrack segregated cardiac precursors has the potential benefit in treating heart failure, thereby paving way in the development of minimal cell manipulation with targeted cell delivery approaches.

Leave a Comment

Your email address will not be published. Required fields are marked *